How can researchers rapidly resolve the miRNA profile of a single cell?

How can researchers rapidly resolve the miRNA profile of a single cell?

Cell Biology

March 13, 2017

Profiling overall microRNA (miRNA) expression in a heterogeneous sample has been the status quo in miRNA research. Although important for understanding the general biology of a system, bulk analysis doesn’t provide information on the individual influence of cell subtypes. This begs the question: how can we effectively profile miRNA from just one cell?

In a newly-released application note, we tested a streamlined end-to-end workflow for miRNA analysis of single cells – starting from isolation of viable single cells, to universal miRNA cDNA synthesis and preamplification, and finally, miRNA profiling and data analysis.

Follow the complete workflow from single-cell isolation to miRNA analysis and download the application note.

 

Improve DNA extraction from diverse soil environments

Improve DNA extraction from diverse soil environments

Cell Biology

It is well known that the gut microbiota contributes to health and disease. Many studies have demonstrated that external factors such as diet, geographical location and host genetics are key contributors. However, the relative contribution of these factors in shaping the gut microbiota is unclear, likely due to large interindividual variation.

February 28, 2017 More +
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