Western blot Protocol from Abcam!

Western blot Protocol from Abcam!

Cytogenetics / Molecular Biology

April 29, 2019

Western blotting uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). 

The gel is placed next to the membrane and application of an electrical current induces the proteins to migrate from the gel to the membrane. The membrane can then be further processed with antibodies specific for the target of interest, and visualized using secondary antibodies and detection reagents.

Abcam offers the western blot protocol that includes solutions and reagents, procedure, and useful links to guide you through your experiment.

Click here to view the western blot protocol!

SDS-PAGE sample preparation from Abcam!

SDS-PAGE sample preparation from Abcam!

Cytogenetics / Molecular Biology

Quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis with Abcam Optiblot SDS-PAGE sample preparation kit!

 

April 23, 2019 More +
Exosomes – small vesicles, big players

Exosomes – small vesicles, big players

Cytogenetics / Molecular Biology

Functional study of exosomes and exoRNAs starts with isolation. Are you still performing time-consuming ultracentrifugation to isolate the contents of exosomes and other extracellular vesicles?

January 11, 2017 More +
Ready to make an enquiry Call us on: + 961 1 39 66 77
Or email us