Revolutionary Next Generation IHC allows quantitative multiplexed images

Revolutionary Next Generation IHC allows quantitative multiplexed images

Anatomy - Pathology / Molecular Diagnostics / Molecular Biology

March 12, 2015

Labelled monoclonal antibodies analysed by mass spectrometry allow for quantitative multiplexing on intact tissue samples. This significant jump in the quantity of proteins detectable on a tissue sample is truly the next generation of IHC.

Recent advances in immunohistochemistry (IHC) techniques have improved their dynamic range by multiple orders of magnitude. This significant jump in the quantity of proteins detectable on a tissue sample is truly the next generation of IHC.

The dynamic range of IHC is 1 log and 2.5 logs for quantitative immunofluorescence (QIF), which measures the relative amount of a target protein in a tissue sample. Mass spectrometry IHC (MSIHC) can reach a value of 5 logs.

Currently there are limitations to IHC due to the restriction of detection techniques, loss of spatial resolution with chromogenic methods, loss of sensitivity with fluorescent methods, heterogeneity and variability in pre-processing of samples.

With the ability to allow multiplexed and directly quantitative imaging of tissue samples, this technique helps to overcome many of the current IHC limitations. It is indeed cutting edge technology for basic and clinical research.

The great advantage of this technique is that multiple monoclonal antibodies for the same protein are analyzed, revealing antibody specificity by directly comparing one antibody to another. It highlights the different specificity of the antibodies for different protein domains as a function of changes in the structure of the protein or post translational modifications.

The technique also has the advantage of allowing subtle changes in multiple proteins to be studied at once, due to the simultaneous use of a high number of heavy metal ions (high plexing capability). Finally, the multiplex advantage allows the use of antibodies against housekeeping proteins for reference and normalization, giving detailed pre-processing information and providing more reproducible and accurate IHC results. This opens the door to IHC as a more trustworthy clinical and diagnostic application.

It is important to note that for both methods the quality of the antibody is crucial. Poorly characterized or cross-reactive antibodies will give non-reproducible results because the interaction between antigen and antibody still has the primary role in the measurement of expression.

We offer RabMAb® products; a range of high quality rabbit monoclonal antibodies that complements this innovative technology. RabMAb products are ideal IHC reagents:

Additionally, we provide high quality rabbit monoclonal antibodies for anatomic pathology (IVD IHC usage).

NEW single-use data loggers

NEW single-use data loggers

Anatomy - Pathology / Molecular Diagnostics / Mole...

Two new single-use USB data loggers for temperature monitoring of temperature sensitive goods during transport: the EBI 330-T30 and the EBI 330-T85. These loggers are ideal for Pharmaceutical or life science shipments where returning a more expensive multi-use logger after a shipment is difficult. All the user must do is: start the logger and place it with the shipment. The receiver simply plugs it into a computer, and is able to read the automatically generated pdf report. There are no further hassles with returning the logger to the sender!

 

February 01, 2013 More +
NEW ELISA kits for the determination of species content in food and feed

NEW ELISA kits for the determination of species content in food and feed

Anatomy - Pathology / Molecular Diagnostics / Mole...

R-Biopharm is proud to announce its partnership with US test kit manufacturer ELISA Technologies. Starting January 1st, R-Biopharm and its worldwide distribution network started to offer the ELISA-TEK™ and MELISA-TEK™ test kits for the detection and identification of species content in food and feed. Another addition to the portfolio is the EZ PANGASISUS™ lateral flow test for the positive identification of pangasius filet

December 20, 2017 More +
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