How can researchers rapidly resolve the miRNA profile of a single cell?

How can researchers rapidly resolve the miRNA profile of a single cell?

Cell Biology

March 13, 2017

Profiling overall microRNA (miRNA) expression in a heterogeneous sample has been the status quo in miRNA research. Although important for understanding the general biology of a system, bulk analysis doesn’t provide information on the individual influence of cell subtypes. This begs the question: how can we effectively profile miRNA from just one cell?

In a newly-released application note, we tested a streamlined end-to-end workflow for miRNA analysis of single cells – starting from isolation of viable single cells, to universal miRNA cDNA synthesis and preamplification, and finally, miRNA profiling and data analysis.

Follow the complete workflow from single-cell isolation to miRNA analysis and download the application note.

 

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