How can researchers rapidly resolve the miRNA profile of a single cell?

How can researchers rapidly resolve the miRNA profile of a single cell?

Cell Biology

March 13, 2017

Profiling overall microRNA (miRNA) expression in a heterogeneous sample has been the status quo in miRNA research. Although important for understanding the general biology of a system, bulk analysis doesn’t provide information on the individual influence of cell subtypes. This begs the question: how can we effectively profile miRNA from just one cell?

In a newly-released application note, we tested a streamlined end-to-end workflow for miRNA analysis of single cells – starting from isolation of viable single cells, to universal miRNA cDNA synthesis and preamplification, and finally, miRNA profiling and data analysis.

Follow the complete workflow from single-cell isolation to miRNA analysis and download the application note.

 

Cubis®High-Capacity Micro Balances: Weigh Minimum Amounts of Sample Directly into Heavy Flasks

Cubis®High-Capacity Micro Balances: Weigh Minimum Amounts of Sample Directly into Heavy Flasks

Cell Biology

With a unique resolution of up to 60 million divisions and a readability of 1 μg, the new high-capacity Cubis® MSA66S micro-analytical balances feature a maximum capacity of 61 g.
Based on this high weighing capacity, you will no longer need to transfer samples and can weigh minimum amounts directly into relatively heavy containers, such as long-necked flasks or HPLC bottles, saving an extra step in your procedure, ensuring that you can use 100% of your sample.

 

 

January 17, 2018 More +
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