Western blot Protocol from Abcam!

Western blot Protocol from Abcam!

Cytogenetics / Molecular Biology

April 29, 2019

Western blotting uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). 

The gel is placed next to the membrane and application of an electrical current induces the proteins to migrate from the gel to the membrane. The membrane can then be further processed with antibodies specific for the target of interest, and visualized using secondary antibodies and detection reagents.

Abcam offers the western blot protocol that includes solutions and reagents, procedure, and useful links to guide you through your experiment.

Click here to view the western blot protocol!

MTP tissue processor

MTP tissue processor

Cytogenetics / Molecular Biology

26 Reasons why MTP tissue processor is on the top of carousel instruments!

October 23, 2015 More +
Optimize your experiment with SimpleStep ELISA® kits from Abcam!

Optimize your experiment with SimpleStep ELISA® kits from Abcam!

Cytogenetics / Molecular Biology

Unlike traditional sandwich ELISA, that take more than three hours to run, SimpleStep ELISA® kits generate data in just 90 minutes, without compromising on performance! SimpleStep ELISA® kits are sandwich ELISA assays that have been developed to be as simple and effective as possible.

 

May 08, 2019 More +
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